Culture of Animal Cells A Manual of Basic Technique and Specialized Applications, Sixth Edition

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition By R. Ian Freshney(auth.)
2010 | 772 Pages | ISBN: 0470528125 | PDF | 87 MB
This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs.PowerPoint slides of the figures as well as other supplementary materials are available at a companion website:www.wiley.com/go/freshney/cellcultureContent: Chapter 1 Introduction (pages 1-10): Chapter 2 Biology of Cultured Cells (pages 11-23): Chapter 3 Laboratory Design, Layout, and Equipment (pages 25-36): Chapter 4 Equipment and Materials (pages 37-56): Chapter 5 Aseptic Technique (pages 57-70): Chapter 6 Safety, Bioethics, and Validation (pages 71-88): Chapter 7 Culture Vessels and Substrates (pages 89-98): Chapter 8 Defined Media and Supplements (pages 99-114): Chapter 9 Serum?Free Media (pages 115-132): Chapter 10 Preparation and Sterilization (pages 133-162): Chapter 11 Primary Culture (pages 163-186): Chapter 12 Subculture and Cell Lines (pages 187-206): Chapter 13 Cloning and Selection (pages 207-225): Chapter 14 Cell Separation (pages 227-237): Chapter 15 Characterization (pages 239-268): Chapter 16 Differentiation (pages 269-278): Chapter 17 Transformation and Immortalization (pages 279-297): Chapter 18 Contamination (pages 299-315): Chapter 19 Cryopreservation (pages 317-334): Chapter 20 Quantitation (pages 335-364): Chapter 21 Cytotoxicity (pages 365-381): Chapter 22 Specialized Cells (pages 383-432): Chapter 23 Stem Cells, Germ Cells, and Amniocytes (pages 433-462): Chapter 24 Culture of Tumor Cells (pages 463-479): Chapter 25 Three?Dimensional Culture (pages 481-495): Chapter 26 Scale?Up and Automation (pages 497-515): Chapter 27 Specialized Techniques (pages 517-532): Chapter 28 Training Programs (pages 533-568): Chapter 29 Problem Solving (pages 569-591): Chapter 30 In Conclusion (page 593):