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CRISPR Cas System Applications in Gene Editing and Beyond

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CRISPR Cas System Applications in Gene Editing and Beyond
Published 08/2022
MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHz, 2 Ch
Genre: eLearning | Language: English + srt | Duration: 72 lectures (5h 45m) | Size: 2.87 GB
Learn about applications of CRISPR in gene editing, gene regulation, ChIP, live-cell imaging, diagnosing diseases etc.


What you'll learn
Introductory overview of basic concepts of Molecular Biology
What is gene editing?
Gene editing tools: Zinc Finger Nucleases (ZFNs) and TALENs and their limitations
DNA repair by Homology Directed Repair (HDR) pathway and Non Homologous End joining (NHEJ) repair
Introduction to CRISPR-Cas system and how it can be used as a gene editing tool?
Components, types and mechanism of CRISPR-Cas system
Protospacer Adjacent Motifs or PAMs
Off target activity of SpCas9 nuclease
Variants of Cas9 nuclease: Cas9 nickase, dead Cas9 (dCas9) and FokI fused catalytically inactive Cas9,
Cas9 nucleases with novel PAM specificities
High Fidelity SpCas9
Cas9 orthologs: SaCas9, NmCas9, CjCas9
Viral vectors for CRISPR delivery: Retroviral vectors, Adenoviral vectors, AAV vectors, etc.
Prime editing: its components, mechanism, applications, advantages and limitations
CRISPR-mediated Base editing: its components, mechanism, applications, advantages and limitations
CRISPR activation or CRISPRa
CRISPR interference or CRISPRi
Epigenetic editing with dCas9
CRISPR-mediated Chromatin Immunoprecipitation (ChIP)
Fluorescence In Situ Hybridization (FISH)
CRISPR-mediated live-cell imaging of genomic loci
Cas12 and Cas13 enzymes for diagnosing infectious diseases
Sherlock CRISPR-Cas13-based diagnostic kit
DETECTOR: CRISPR-Cas12-based diagnostic kit
FELUDA: CRISPR-Cas19-based diagnostic kit
Requirements
Knowledge of basic biology
Motivation to learn
Description
Gene editing with the CRISPR-Cas system has been called "one of the biggest technologies of the decade" for its applications in medicine, agriculture, and much more. And in this course we will delve deep into the details of gene editing and why it is being seen as a promising strategy for the treatment of numerous genetic diseases like sickle cell disease, cystic fibrosis, etc to the generation of engineered crops, wiping out of malaria causing mosquitoes and others. You will get advanced learning of how CRISPR functions as a gene-editing tool, its comparison to other gene editing tools like Zinc Finger Nucleases, TALENs, etc, components of the CRISPR-Cas system, and the different ways in which CRISPR can be applied to healthcare, agriculture, and many other industries.
The beauty of the CRISPR system is that its applications extend much further beyond just gene editing.
For instance, the CRISPR-Cas system can be used as a powerful tool to regulate gene expression. In this course, we will learn how scientists have been modifying the CRISPR system to reversibly activate or silence genes and edit the epigenome in previously unimaginable ways. This can open the doors to new treatment options and a better understanding of human pathogenesis.
This course will cover how fluorescent proteins can be attached to the components of CRISPR-Cas to detect and locate the positions of specific DNA sequences on the chromosomes. This, in turn, can be useful to visualize the 3D architecture of the genome or to know various chromosomal abnormalities, including deletions, duplications, and translocations that influence cell function in normal and diseased states.
Additionally, we will also discuss, how using CRISPR, the researchers have expanded chromatin immunoprecipitation or ChIP to identify proteins associated with a particular genomic sequence.
Further in this course, we will talk about how scientists have harnessed the CRISPR-Cas technology for diagnosing infectious diseases including COVID-19.
The limitations and challenges of the CRISPR-Cas system have also been discussed. Additionally, this course will provide a detailed discussion on CRISPR-mediated precise gene editing techniques like prime editing and base editing, that can revolutionize the treatment of genetic diseases while limiting the negative effects of the CRISPR-Cas system. For precise gene editing and non-gene editing applications, several variants of Cas9 nuclease have been engineered. In this course, you will get a thorough understanding of these variants, their engineering, and their significance.
This course is an invaluable resource for medical students, doctors, and students of disciplines like biotechnology, biology, immunology, genetics, molecular biology, cell biology, and bioinformatics. In the course, the most challenging concepts are presented in a simple and palatable format using animations and graphics.
Who this course is for
The course is designed for medical students, doctors, researchers and students of biology, biotechnology, genetics, molecular biology, immunology, cell biology and bioinformatics disciplines
Anyone who has CRISPR-Cas system as a subject in their curriculum
Anyone looking for career opportunities in the field of CRISPR-Cas technology and genomics
Anyone looking to study biology at college or university and wants quick, to-the-point knowledge about CRISPR technology
Newly qualified teachers who need a good resource on CRISPR-Cas technology
Anyone who wants to get an in-depth knowledge about CRISPR-Cas system and their applications
Homepage
https://www.udemy.com/course/crispr-cas-system-applications-in-gene-editing-and-beyond/





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